AJSM Click here for details!
HOME HELP CONTACT US SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Sign In to gain access to subscriptions and/or personal tools.
First published on August 16, 2004, doi:10.1177/0363546503262179
This version was published on October 1, 2004
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
32/7/1613    most recent
0363546503262179v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Request Reprints
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via ISI Web of Science (8)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Seneviratne, A.
Right arrow Articles by Hannafin, J. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Seneviratne, A.
Right arrow Articles by Hannafin, J. A.
Related Collections
Right arrow Injury
Right arrow Animal studies
Right arrow Female Athletes
The American Journal of Sports Medicine 32:1613-1618 (2004)
© 2004 American Orthopaedic Society for Sports Medicine

The Effect of Estrogen on Ovine Anterior Cruciate Ligament Fibroblasts

Cell Proliferation and Collagen Synthesis

Aruna Seneviratne, MD*, Erik Attia*, Riley J. Williams, MD*,{dagger}, Scott A. Rodeo, MD* and Jo A. Hannafin, MD, PhD*,{ddagger}

From the * Hospital for Special Surgery, New York, New York, and {dagger} Cornell University, New York, New York

{ddagger} Address correspondence to Jo A. Hannafin, MD, PhD, Hospital for Special Surgery, 535 E. 70th Street, New York, NY 10021 (e-mail: hannafinj{at}hss.edu).

Abstract: Estrogen has been implicated as a causal factor for anterior cruciate ligament injuries in women. Studies have demonstrated a decrease in anterior cruciate ligament fibroblast proliferation and collagen synthesis at supraphysiologic levels of estrogen in a rabbit model.

Hypothesis: The authors hypothesized that physiologic levels of estrogen would have no significant effect on anterior cruciate ligament fibroblast proliferation and collagen synthesis in an ovine model.

Methods: Anterior cruciate ligament fibroblasts were isolated from sheep knees using routine cell culture methods. The cells were exposed to 17ß-estradiol at physiologic concentrations of 2.2, 5, 15, 25, 250, and 2500 pg/ml. Cell proliferation was determined by cell counts on days 4 and 6. Collagen synthesis was determined by 3H-proline incorporation on day 4. Immunohistochemistry was performed to detect estrogen receptors.

Results: Immunohistochemistry demonstrated the presence of estrogen receptors in ovine anterior cruciate ligament fibroblasts. There was no significant difference in anterior cruciate ligament fibroblast proliferation or collagen synthesis regardless of 17ß-estradiol concentration.

Conclusions: Based on results of this study, and given the low turnover of collagen in ligaments, it is unlikely that a 2- to 3-day per month increase in circulating estrogen would result in rapid, clinically significant alterations in material properties of the anterior cruciate ligament in vivo. The etiology of noncontact anterior cruciate ligament injuries is complex and multifactorial in nature, meriting further investigation.

Key Words: estrogen • collagen synthesis • anterior cruciate ligament (ACL) • cell proliferation




This article has been cited by other articles:


Home page
 Am J Sports MedHome page
K. Knobloch, L. Schreibmueller, R. Meller, K. H. Busch, M. Spies, and P. M. Vogt
Superior Achilles Tendon Microcirculation in Tendinopathy Among Symptomatic Female Versus Male Patients
Am. J. Sports Med., March 1, 2008; 36(3): 509 - 514.
[Abstract] [Full Text] [PDF]

Home page
 Am J Sports MedHome page
L. Y. Griffin, M. J. Albohm, E. A. Arendt, R. Bahr, B. D. Beynnon, M. DeMaio, R. W. Dick, L. Engebretsen, W. E. Garrett Jr, J. A. Hannafin, et al.
Understanding and Preventing Noncontact Anterior Cruciate Ligament Injuries: A Review of the Hunt Valley II Meeting, January 2005
Am. J. Sports Med., September 1, 2006; 34(9): 1512 - 1532.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
S. J. Warden, L. K. Saxon, A. B. Castillo, and C. H. Turner
Knee ligament mechanical properties are not influenced by estrogen or its receptors
Am J Physiol Endocrinol Metab, May 1, 2006; 290(5): E1034 - E1040.
[Abstract] [Full Text] [PDF]

Home page
 JBJSHome page
L. J. Rubino III and M. D. Miller
What's New in Sports Medicine
J. Bone Joint Surg. Am., February 1, 2006; 88(2): 457 - 468.
[Full Text] [PDF]

Home page
 Am J Sports MedHome page
T. E. Hewett, G. D. Myer, and K. R. Ford
Anterior Cruciate Ligament Injuries in Female Athletes: Part 1, Mechanisms and Risk Factors
Am. J. Sports Med., February 1, 2006; 34(2): 299 - 311.
[Abstract] [Full Text] [PDF]


HOME HELP CONTACT US SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 2004 by the American Orthopaedic Society for Sports Medicine.