HOME HELP CONTACT US SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:
Sign In to gain access to subscriptions and/or personal tools.

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in ISI Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Permissions Request Reprints Citing Articles Citing Articles via HighWire Citing Articles via ISI Web of Science (9) Citing Articles via Google Scholar Google Scholar Articles by Tomiya, A. Articles by Kokubun, S. Search for Related Content PubMed PubMed Citation Articles by Tomiya, A. Articles by Kokubun, S. Related Collections Animal studies Histology Muscle

Myofibers Express IL-6 After Eccentric Exercise

Akihito Tomiya, MD, PhD^*,, Toshimi Aizawa, MD, PhD, Ryoichi Nagatomi, MD, PhD, Hiroomi Sensui, MS and Shoichi Kokubun, MD, PhD

From the Department of Orthopaedic Surgery, Tohoku University School of Medicine, Sendai, Japan, and the Department of Medicine & Exercise in Sports & Exercise, Tohoku University Graduate School of Medicine, Sendai, Japan

^* Address correspondence to Akihito Tomiya, MD, Department of Orthopaedic Surgery, Tohoku University School of Medicine, 1-1 Seiryomachi, Aoba-ku, Sendai, 980-8574, Japan.

Background: Interleukin (IL)-6 is locally produced in skeletal muscles and shows a remarkable increase in plasma after eccentric exercises.

Objective: To elucidate the cell types in the muscles responsible for IL-6 production after eccentric exercises.

Study Design: Controlled laboratory study.

Methods: An eccentric contraction model was made using electrical stimulation. The authors investigated the muscle damage and regeneration processes after eccentric exercises histologically, and the cell types expressing IL-6 and its subcellular compartimentalization with time immunohistochemically after eccentric exercises.

Results: Swollen myofibers were detected from 8 hours to 3 days after exercises. Disrupted myofibers were detected from 24 hours to 7 days, with a peak of 3 days. IL-6 was detected only in the cytoplasm of myofibers until 12 hours; thereafter, it was found in the inflammatory cells and proliferating satellite cells as well. The swollen myofibers were negatively stained for IL-6. The positive ratios of IL-6 in myofibers immediately increased after exercises, peaked in 12 hours, and then decreased.

Conclusions: After eccentric exercises, IL-6 expression increased in myofibers preceding the disruption of myofibers. IL-6 might be closely related to muscle damage caused by strenuous exercises.

Key Words: skeletal muscle • eccentric exercise • interleukin (IL)-6 • muscle damage

This article has been cited by other articles:

				M. P. Miles, J. M. Andring, S. D. Pearson, L. K. Gordon, C. Kasper, C. M. Depner, and J. R. Kidd Diurnal variation, response to eccentric exercise, and association of inflammatory mediators with muscle damage variables J Appl Physiol, February 1, 2008; 104(2): 451 - 458. [Abstract] [Full Text] [PDF]

				M. K. Trenerry, K. A. Carey, A. C. Ward, and D. Cameron-Smith STAT3 signaling is activated in human skeletal muscle following acute resistance exercise J Appl Physiol, April 1, 2007; 102(4): 1483 - 1489. [Abstract] [Full Text] [PDF]

				M. D. Carmichael, J. M. Davis, E. A. Murphy, A. S. Brown, J. A. Carson, E. P. Mayer, and A. Ghaffar Role of brain IL-1beta on fatigue after exercise-induced muscle damage Am J Physiol Regulatory Integrative Comp Physiol, November 1, 2006; 291(5): R1344 - R1348. [Abstract] [Full Text] [PDF]