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Joint Resurfacing with Cartilage Grown in Situ from Cell-Polymer Structures

Department of Surgery, Children's Hospital and Harvard Medical School, Boston, Massachusetts

WooSeob Kim, MD, PhD

Department of Surgery, Children's Hospital and Harvard Medical School, Boston, Massachusetts

Betsy Schloo, MD

Department of Surgery, Children's Hospital and Harvard Medical School, Boston, Massachusetts

Joseph Upton, MD

Department of Surgery, Children's Hospital and Harvard Medical School, Boston, Massachusetts

Joseph P. Vacanti, MD

Department of Surgery, Children's Hospital and Harvard Medical School, Boston, Massachusetts

We tested the potential of a new technology developed in our laboratory to create new hyaline cartilage for re surfacing distal femoral joint surfaces of New Zealand White rabbits that had been surgically denuded of ar ticular cartilage. We removed hyaline cartilage from the patellar groove of the distal femurs in 24 rabbits. Chon drocytes isolated from the excised cartilage of 12 of these rabbits (experimentals) were seeded onto syn thetic biocompatible, biodegradable polymers com posed of polyglycolic acid. The cells were labeled in vitro with a thymidine analog, BrdU (5-bromo-2' deoxyuridine). After 1 week in vitro, the cell-polymer structures were implanted onto the denuded surfaces of 12 defects made in the hyaline cartilage of the con tralateral knees of the experimental animals. Twelve control animals received either no implants or implants not containing cells on similar surgical defects. After 7 weeks, we found evidence of new cartilage growth in 11 of the 12 experimental animals and virtually no new car tilage formation in any of the animals in either control group. Immunohistochemical analysis demonstrated the presence of BrdU-labeled chondrocytes in repre sentative specimens.

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