HOME HELP CONTACT US SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:
Sign In to gain access to subscriptions and/or personal tools.

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Alert me to new issues of the journal Download to citation manager Request Permissions Request Reprints Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Williams, J. A. Articles by Thonar, E. J.-M.A. Search for Related Content PubMed Articles by Williams, J. A. Articles by Thonar, E. J.-M.A.

Early osteophyte formation after chemically induced articular cartilage injury

Department of Anatomy, Rush Medical College, Chicago, Illinois, Department of Internal Medicine, Rush Medical College, Chicago, Illinois

Eugene J.-M.A. Thonar, PhD

Department of Internal Medicine, Rush Medical College, Chicago, Illinois, Department of Biochemistry, Rush Medical College, Chicago, Illinois

We examined the early changes that follow injection of sodium iodoacetate (IA) into the guinea pig knee joint, using Safranin-O (SO) to stain the proteoglycan (PG)- rich matrix and the incorporation of Na ³⁵ SO₄ to deter mine which cells have maintained, lost, or developed the ability to synthesize significant amounts of PGs. Within 24 hours following injection of IA, articular chon drocytes exhibited a marked loss in ability to synthesize PGs; this inhibition was complete after 2 weeks. Focal loss of SO staining of the interterritorial matrix was noted after 24 hours, progressing to a complete loss of staining after 2 weeks. In sharp contrast to the degenerative changes in the habitually loaded articular cartilage, cells at the chondrosynovial junction began incorporating Na ³⁵ SO₄ and producing a matrix that stained with SO as early as 72 hours following injection. With time, this resulted in the development of prominent osteophytic cartilage that involved bone remodeling beneath the overlying cartilaginous cap after 3 weeks.